CSIR Biochemistry MCQ question part - 3

21. DNA sequencing is done on a sequencing gel, which is
(a) SDS-PAGE (b) Urea-PAGE
(c) Native PAGE (d) Agarose

22. The digestion of lipid takes place in small intestine by lipases, However, it does not
occur till bile fluid mixes with the food. This mixing up of food with bile juice is
essential for digestion of lipids because
(a) lipases present in the intestine require another coenzyme present in the bile
juice for its functioning
(b) Emulsification is must for fats to be digested and for lipases to act upon lipids
(c) lipase can only act at alkaline pH
(d) None of the above

23. Mark the Incorrect statement:
(a) Endocrine signaling is relatively slow because it depends on diffusion and blood
(b) If the same hormone has different effects on different target organs, the receptor
are usually different
(c) Water soluble hormones interact with cell surface receptors, whereas lipid soluble
hormones usually bind to intracellular receptors
(d) The specificity of hormone signaling depends on responsive cells possessing
receptors that bond the hormone tightly by means of multiple weak bonds.

24. Silencers are located to upstream of transcription start site and function-
(a) in both the orientation (b) in forward orientation
(c) in reverse orientation (d) None of the above

25. The renaturation of the genome is a random collision of complementary sequences
and follows
(a) first order kinetics (b) second order kinetics
(c) third order kinetics (d) None of the above

26. Which one of the following electrophoresis dependents least on the charge of the
(a) Free zone capillary electrophoresis
(b) Gel electrophoresis
(c) SDS-polycrylamide gel electrophoresis
(d) Isoelectric focussing

27. Which one of the following compound has the highest group transfer potential for
phosphate ?
(a) Glucose-6-phosphate (b) Fructose-1, 6-diphosphate
(c) 2-phosphoenolpyruvate (d) Adenosine triphosphate

28. Which one of the following sets of glycolytic enzymes is allosterically regulated?
(a) Glucokinase, phosphofructokinase and pyruvate kinase
(b) Hexokinase, aldolase and pyruvate kinase
(c) Phosphofructokinase, enolase and pyruvate kinase
(d) Hexokinase, phosphofructokinase and pyruvate kinase

29. Which one of the following compounds is required for acetyl coenzyme A (acetyl
CoA)to enter the citric acid cycle?
(a) Isocitrate (b) Malate
(c) Oxaloacetate (d) Pyruvate

30. Which one of the following DNA polymerase is essential for both the replication and
repair of DNA?
(a) DNA polymerase I (b) DNA polymerase II
(c) DNA polymerase III (d) DNA polymerase d

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