Biotechnology Exam Questions part -2

11. Plastome is

a)Plasmalemma protein

b)A type of plasmid

c)An organellar genome

d)None of the above

Ans: plastome: Genetic material of plastid like Chloroplast

12. Which of the following process require energy

a) ligation

b) restriction digestion

c) hybridization

d) transformation

Ans:a

Ligation: sealing of single stranded nicks or breaks by ligase enzyme. The process requires ATP and NADP⁺.

13) Enhanced axillary branching for multiple shoot production is promoted by

a)2,4-D

b) Abscisic acid

c) BA (Benzyl adenine)

d) Gibberellic acid

Ans:a

2,4-D is an auxin and auxin is responsible for sustaining apical dominance there by promoting axillary branching

14. Viral replication within cells is inhibited by

a) IL-4

b) IL-1

c) IFN alpha

d) TNF alpha

Ans:c

Interferons: are antiviral agents (proteins) secreted by virus infected cells and induces a virus resistant state to the surrounding cells by inhibiting its replication.

15. In the course of cell cycle, the level of the protein cyclin abruptly falls during

a) G1phase

b) G2 phase

c) S phase

d) M phase

Ans:d

16. Protein binding regions of DNA are identified by one of the following techniques

a) Finger printing

b) Foot printing

c) Southern blotting

d) Western blotting

  Ans: (b) Foot Printing

• Foot Printing: The identification of a protein-binding site on a DNA molecule by determining which phosphodiester bonds are protected from cleavage by DNase I.
• Southern Blotting: The technique used for identifying specific DNA fragment using labelled probe.

17.Si RNA (s) interfere at

a) Transcriptional level

b) Post -transcriptional level

c) DNA replication level

d)Translational level

Ans: (b) Post -transcriptional level

18. Plant secondary metabolites

a) Help to increase the growth rate of plant

b) Help in plant reproduction processes

c) Provide defence mechanisms against microbial attack

d) Make the plant susceptible to unfavourable conditions

Ans: (c) Provide defence mechanisms against microbial attack

19. Mobile genetic elements present in human genome are

P) LINES

Q) SINES

R) P elements

S) IS elements

(a) Q, R (b) P, Q  (c) P, R (d) Q, S

Ans: (b) P, Q

20. Meristems escape virus invasion because

a)Vascular system is absent in the meristem

b) of low metabolic activity in the meristem

c) of low endogenous auxin level

d) the virus inactivating system has low activity in the meristem

Ans: b) of low metabolic activity in the meristem