Biotechnology Exam Questions part -4

21. The enzyme that can be used in 5’end labeling of DNA are
P) Alkaline phosphatase
Q) DNA ligase
R) Terminal transferase
S) Polynucleotide kinase
(a) P, S (b) R, Q (c) P, R (d) R, S
Ans: (a) P, S
22. Identify the natural plant growth regulators from the following list
P) Zeatin
Q) Benzylamino purine (BAP)
R) Indole Acetic Acid (IAA)
S)  2,4-Dichlorophenoxyacetic acid (2,4-D)
(a)P, Q (b) Q, S (c) P, R(d) R,S
Ans: c) P, R
23. Cells of meristemoid are best described as
a) differentiated and non dividing
b) Dedifferentiated and dividing
c) differentiated and dividing
d) dedifferentiated and non dividing
Ans: c) differentiated and dividing
24. Ultra filtration process cannot be used for
a)Fractionation of Proteins
c) Harvesting of cells
d)Selective removal of solvents
Ans: c) Harvesting of cells
25. The number of replicons in a typical mammalian cell is
a) 40-200
b) 400
c) 1000-2000
d) 50000-100000
Ans: a) 40-200
26. What product will result from complete hydrolysis of soluble dextran
a) Sucrose only
b) Fructose only
c) Glucose &Fructose only
d) Glucose only
Ans: d) Glucose only
27. The mobility of DNA in agarose gel electrophoresis is solely based on its
a) Charge
b) Conformation
c) Size
d) None of these
Ans: c) Size
28. Which of the following fluorescent probes is used to monitor the progress of amplification of Real Time PCR?
a) SYBR Green
c) Cyan Blue
d) Rhodamine
Ans: b) FITC
29. Expression of which of the following reporter genes does not require addition of specific substrate for detection
a) Luciferase
b) β- Glucuronidase
c) β-Glucosidase
d) Green Fluorescent Protein (GFP)
Ans: a) Luciferase
30. Zinc fingers are characteristic of
(a) Blood clotting Proteins
(b) RNA Chaperones
(c) DNA binding proteins
(d) Lysosomal hydrolyses
Ans: DNA binding proteins
  • Chaperons: proteins that assist in proper folding of proteins.