21. The enzyme that can be used in 5’end labeling of DNA are
P) Alkaline phosphataseQ) DNA ligaseR) Terminal transferaseS) Polynucleotide kinase
(a) P, S (b) R, Q (c) P, R (d) R, S
Ans: (a) P, S
22. Identify the natural plant growth regulators from the following list
P) ZeatinQ) Benzylamino purine (BAP)R) Indole Acetic Acid (IAA)S) 2,4-Dichlorophenoxyacetic acid (2,4-D)
(a)P, Q (b) Q, S (c) P, R(d) R,S
Ans: c) P, R
23. Cells of meristemoid are best described as
a) differentiated and non dividingb) Dedifferentiated and dividingc) differentiated and dividingd) dedifferentiated and non dividing
Ans: c) differentiated and dividing
24. Ultra filtration process cannot be used for
a)Fractionation of Proteinsb)Desaltingc) Harvesting of cellsd)Selective removal of solvents
Ans: c) Harvesting of cells
25. The number of replicons in a typical mammalian cell is
a) 40-200b) 400c) 1000-2000d) 50000-100000
Ans: a) 40-200
26. What product will result from complete hydrolysis of soluble dextran
a) Sucrose onlyb) Fructose onlyc) Glucose &Fructose onlyd) Glucose only
Ans: d) Glucose only
27. The mobility of DNA in agarose gel electrophoresis is solely based on its
a) Chargeb) Conformationc) Sized) None of these
Ans: c) Size
28. Which of the following fluorescent probes is used to monitor the progress of amplification of Real Time PCR?
a) SYBR Greenb) FITCc) Cyan Blued) Rhodamine
Ans: b) FITC
29. Expression of which of the following reporter genes does not require addition of specific substrate for detection
a) Luciferaseb) β- Glucuronidasec) β-Glucosidased) Green Fluorescent Protein (GFP)
Ans: a) Luciferase
30. Zinc fingers are characteristic of
(a) Blood clotting Proteins(b) RNA Chaperones(c) DNA binding proteins(d) Lysosomal hydrolyses
Ans: DNA binding proteins
- Chaperons: proteins that assist in proper folding of proteins.
