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Consider two proteins- a transcription factor (nuclear protein) and a hormone receptor (membrane-spanning protein).
Protein 1: transcription factor NH3--------------------------------COO-
Protein 2: Hormone Receptor NH3-------------------------------- COO-
a) What is the difference between protein localization sequences in proteins 1 and 2 such that they are targeted to different cell compartments?
b) Indicate where you would expect to find proteins 1 and 2 in a cell that has a mutated signal recognition particle (SRP). Explain why proteins would be found in these locations.
c) Indicate the correct sequence of events that will lead to the proper localization of proteins 1 and 2.
1.Protein synthesis is blocked by SRP
2.After removal of signal sequence, growing polypeptide enters ER
3.Glycosylation occurs
4.Protein is anchored in plasma membrane
5.Translation of polypeptide chain starts in the cytoplasm on the ribosome
6.Protein synthesis resumes
7.Protein is transported to Golgi in a vesicle
8.SRP attaches to a specific receptor on the ER
9.Growing polypeptide becomes embedded in ER membrane.
10.Vesicle harboring protein buds off Golgi
11.Protein enters nucleus
12.Signal sequence is removed
13.SRP binds signal sequence
14.Vesicle fuses with the plasma membrane
15.Signal sequence binds to receptor on nucleus opening channel
PROTEIN 1: Write correct sequence of event here for Nuclear protein here (for example 1,5,15.......)
PROTEIN 2:Write correct sequence of event here for Hormon receptor protein here (for example 1,5,15.......)
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ICMR JRF study material - 2010 ICMR Question papers
ICMR JRF is a relatively tough compare to other life science competitive exam like CSIR/GATE But those exams has model question paper, old question paper. Problem with ICMR JRF exam is there no previous year question paper. Here for the benefit of fellow Aspirants and all future ICMR JRF Aspirants we collected ICMR questions from various sources, students,institution,piled into a single pdf
Presented ICMR question is completely based on memory from ICMR JRF exam held on July 2010.
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ICMR JRF 2010 question paper
CSIR NET JRF Second paper question practice
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1.Name two applied molecular genetic techniques that depend on the formation of RNA-DNA hybrids.
2. Why does adding NaCl to a DNA solution increase the Tm of a DNA duplex? How does increasing the NaCl concentration affect the "specificity" of a hybridization reaction?
3. How could you take advantage of the Cot phenomenon to functionally "inactivate" highly repetitive DNA sequences from a DNA probe that contains both unique and repeated sequences?
4. Is it possible to ligate a DNA fragment cleaved with BamHI to a heterologous fragment that had been cleaved with the restriction enzyme BglII?. Can the ligated DNA fragments be cleaved again with both BamHI and BglII? Explain.
5. Why is formaldehyde used to denature RNA in a Northern blot, isn't RNA single stranded to begin with? Why can't NaOH be used to denature RNA in the Northern blotting procedure as it is in the Southern Blotting procedure?
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1.Name two applied molecular genetic techniques that depend on the formation of RNA-DNA hybrids.
2. Why does adding NaCl to a DNA solution increase the Tm of a DNA duplex? How does increasing the NaCl concentration affect the "specificity" of a hybridization reaction?
3. How could you take advantage of the Cot phenomenon to functionally "inactivate" highly repetitive DNA sequences from a DNA probe that contains both unique and repeated sequences?
4. Is it possible to ligate a DNA fragment cleaved with BamHI to a heterologous fragment that had been cleaved with the restriction enzyme BglII?. Can the ligated DNA fragments be cleaved again with both BamHI and BglII? Explain.
5. Why is formaldehyde used to denature RNA in a Northern blot, isn't RNA single stranded to begin with? Why can't NaOH be used to denature RNA in the Northern blotting procedure as it is in the Southern Blotting procedure?
Try to answer this question yourself and you can cross check your answer at
Click here to know the answer
CSIR NET JRF Second paper question practice -3
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Several changes occur during cancer progression. Number each of the following events to indicate their most likely order of occurrence, with 1 indicating the earliest stage and 6 indicating the most advanced stage.
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Several changes occur during cancer progression. Number each of the following events to indicate their most likely order of occurrence, with 1 indicating the earliest stage and 6 indicating the most advanced stage.
Submit your answer here
CSIR NET JRF Second paper question practice -2
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Above is shown a picture of a replication bubble.Answer the Question below
(a) The primer 5’-GAA-3’ is being used to replicate this piece of DNA. Would that primer anneal to the upper strand of DNA in the picture or the lower strand?
(b) Write what the product of DNA replication would be if the first five nucleotides had been added onto this primer by DNA polymerase. Label the 5’ and 3’ ends. Make sure to include the primer.
(c) Would that strand that you have drawn in part (b) be a leading strand or a lagging
strand?
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Above is shown a picture of a replication bubble.Answer the Question below
(a) The primer 5’-GAA-3’ is being used to replicate this piece of DNA. Would that primer anneal to the upper strand of DNA in the picture or the lower strand?
(b) Write what the product of DNA replication would be if the first five nucleotides had been added onto this primer by DNA polymerase. Label the 5’ and 3’ ends. Make sure to include the primer.
(c) Would that strand that you have drawn in part (b) be a leading strand or a lagging
strand?
Write your answer Here , we will improve your answer
CSIR NET JRF Second paper question Practice
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1. Where in a eukaryotic cell do you think you would find the following proteins residing? Be as specific as you can in terms of subcellular location.
(a) an enzyme whose substrate is DNA
(b) an enzyme that catalyzes protein synthesis
(c) a protein that allows ions to pass in and out of the cell
(d) a protein that acts as a receptor for extracellular cell-cell communication molecules that are large and hydrophilic
(e) a protein that forms a channel through which RNAs can be exported into the
cytoplasm
(f) an extracellular matrix protein
(g) a protein that adds carbohydrate groups to proteins destined for the outside of the cell
(h) a protein that synthesizes ATP
(i) the cytoskeletal protein actin
(j) The major cytoskeletal protein actin is translated as a single polypeptide subunit.
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1. Where in a eukaryotic cell do you think you would find the following proteins residing? Be as specific as you can in terms of subcellular location.
(a) an enzyme whose substrate is DNA
(b) an enzyme that catalyzes protein synthesis
(c) a protein that allows ions to pass in and out of the cell
(d) a protein that acts as a receptor for extracellular cell-cell communication molecules that are large and hydrophilic
(e) a protein that forms a channel through which RNAs can be exported into the
cytoplasm
(f) an extracellular matrix protein
(g) a protein that adds carbohydrate groups to proteins destined for the outside of the cell
(h) a protein that synthesizes ATP
(i) the cytoskeletal protein actin
(j) The major cytoskeletal protein actin is translated as a single polypeptide subunit.
Try to answer your before checking the answer
For Answer Click here
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